mouse lemur platform offers the following services and equipments :
and maintenance of mouse lemur (Microcebus murinus) under
controlled captive conditions.
of animals of high genetic diversity, of defined age and gender.
Continuous follow up of the animals from birth to death and
integration of the biological measures in an ACCESS data base.
of biological rhythms (locomotor activity, body temperature,
sleep-wake cycle) by wireless continuous recording.
exploration by indirect calorimetry, glycemia and cetonemia measures,
oral glucose tolerance test, insulin sensitivity. Measurements of
blood and urine metabolites (ELISA kits): cholesterol, triglycerides,
testosterone, melatonin, cortisol, etc
up for neuro-functional exploration (EEG methodology).
in nutritional challenge protocols (calorie restriction,
micronutriment supplementation such as polyphenols, omega-3).
phenotyping by infra-red camera recording and motor and behavioural
tests for assessment of anxiety, mnesic performances (spatial,
working and associative memories), motor coordination, physical
strength, moving speed, jump and endurance).
for chemical and gaz anesthesia for surgery with continuous cardiac
and respiratory monitoring (telemetric device implantation,
in protocols of pharmacological substances, of nutritional challenges
of experimental protocols in controlled conditions of ambient
temperature and photoperiodic cycles in ventilated climatic chambers.
for telemetry recording of locomotor activity and body temperature;
for telemetry recording of cerebral electric activity and sleep/wake
cycle by electroencephalography (EEG) and electromyography (EMG);
for indirect calorimetry for real time continuous recording of oxygen
consumption and carbon dioxide production in metabolic cages;
for enzymoimmunologic assays (ELISA);
for immunohistochemistry (fluorescent optic microscopy).
for behavioural and motor performance assessment using videotracking.
for gas anaesthesia.
for night behavioural recording (infra-red cameras).
for molecular biology (DNA/RNA extraction, PCR).